Cell Lines For Fusion Partners For T Cell Hybridomas

Abstract

These cell lines can be used as fusion partners for generating mouse T cell hybridomas. The antigen specific activation response of the T cell hybridomas generated by these cell lines can be measured using either conventional IL-2 assay or assays for the beta-galactosidase enzymatic activity which is specifically induced in these hybridomas. The ability to measure T cell activation using the beta-galactosidase activity is unique to the hybridomas generated with these fusion partners and allows detection of activated T cells either at a single cell level or in bulk-culture. The beta-galactosidase inducible T cell hybridomas can be used to identify unknown T cell stimulating antigens, receptor ligands, as well as unknown mechanisms of MHC class I and MHC class II antigen processing pathways.

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